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| Registros recuperados: 11 | |
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| Brandt, Miriam; Trouche, Blandine; Henry, Nicolas; Liautard-haag, Cathy; Maignien, Lois; De Vargas, Colomban; Wincker, Patrick; Poulain, Julie; Zeppilli, Daniela; Arnaud-haond, Sophie. |
| The abyssal seafloor covers more than 50% of planet Earth and is a large reservoir of still mostly undescribed biodiversity. It is increasingly targeted by resource-extraction industries and yet is drastically understudied. In such remote and hard-to-access ecosystems, environmental DNA (eDNA) metabarcoding is a useful and efficient tool for studying biodiversity and implementing environmental impact assessments. Yet, eDNA analysis outcomes may be biased toward describing past rather than present communities as sediments contain both contemporary and ancient DNA. Using commercially available kits, we investigated the impacts of five molecular processing methods on eDNA metabarcoding biodiversity inventories targeting prokaryotes (16S), unicellular... |
| Tipo: Text |
Palavras-chave: Environmental metabarcoding; RNA versus DNA; Extracellular DNA; Deep-sea biodiversity; Benthic ecology; Biomonitoring; Method testing. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00630/74165/73774.pdf |
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| Vanreusel, Ann; Andersen, Ann C.; Boetius, Antje; Connelly, Douglas; Cunha, Marina R.; Decker, Carole; Hilario, Ana; Kormas, Konstantinos Ar.; Maignien, Lois; Olu, Karine; Pachiadaki, Maria; Ritt, Benedicte; Rodrigues, Clara; Sarrazin, Jozee; Tyler, Paul; Van Gaever, Saskia; Vanneste, Heleen. |
| During the European Commission's Framework Six Programme, HERMES, we investigated three main areas along the European margin, each characterized by the presence of seep-related structures exhibiting different intensity of activity and biological diversity. These areas are: (1) the Nordic margin with the Hakon Mosby mud volcano and many pockmarks, (2) the Gulf of Cadiz, and (3) the eastern Mediterranean with its hundreds of mud volcanoes and brine pool structures. One of the main goals of the HERMES project was to unravel the biodiversity associated with these seep-associated ecosystems, and to understand their driving forces and functions, using an integrated approach. Several multidisciplinary research cruises to these three areas provided evidence of... |
| Tipo: Text |
Palavras-chave: Mosby mud volcano; Gulf of mexico; Cadiz ne atlantic; Deep sea fan; Community structure; Microbial communities; Fluid flow; Mediterranean sea; Carbonate crusts; Norwegian sea. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6375.pdf |
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| Dalmasso, Cécile; Oger, Philippe; Courtine, Damien; Georges, Myriam; Takai, Ken; Maignien, Lois; Alain, Karine. |
| We report the genome sequence of Thermococcus superprofundus strain CDGST, a new piezophilic and hyperthermophilic member of the order Thermococcales isolated from the world’s deepest hydrothermal vents, at the Mid-Cayman Rise. The genome is consistent with a heterotrophic, anaerobic, and piezophilic lifestyle. |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00345/45616/45237.pdf |
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| Cao, Junwei; Maignien, Lois; Shao, Zongze; Alain, Karine; Jebbar, Mohamed. |
| The complete genome sequence of Desulfovibrio indicus J2(T), a member of the family Desulfovibrionaceae, consists of 3,966,573-bp in one contig and encodes 3,461 predicted genes, 5 noncoding RNAs, 3 rRNAs operons, and 52 tRNA-encoding genes. The genome is consistent with a heterotrophic, anaerobic lifestyle including the sulfate reduction pathway. |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00601/71311/69740.pdf |
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| Trigodet, Florian; Larché, Nicolas; Morrison, Hilary G.; Maignien, Lois; Thierry, Dominique. |
| The ennoblement of stainless steel (e.g., the increase of open circuit potential [OCP]) is associated with bacterial colonization. This increases the risk of localized corrosion as the critical pitting/crevice potential can be overcome, especially for lower grade stainless steel. In this study, we assessed the influence of dissolved oxygen content (DOC) on the crevice corrosion of duplex and super duplex stainless steels. In addition, we used DNA amplicon sequencing to identify the bacteria most likely associated with the ennoblement. Above approximately 100 parts per billion (ppb) of dissolved oxygen, the ennoblement of OCP was observed leading to an increased risk of localized corrosion. Below approximately 100 ppb of dissolved oxygen, no ennoblement... |
| Tipo: Text |
Palavras-chave: 16S rRNA gene; Electroactive bacteria; Ennoblement; Microbial ecology; Natural seawater; Stainless steel. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00588/70026/67934.pdf |
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| Lemonnier, Clarisse; Perennou, Morgan; Eveillard, Damien; Fernandez-guerra, Antonio; Leynaert, Aude; Marié, Louis; Morrison, Hilary G.; Memery, Laurent; Paillard, Christine; Maignien, Lois. |
| Ocean frontal systems are widespread hydrological features defining the transition zone between distinct water masses. They are generally of high biological importance as they are often associated with locally enhanced primary production by phytoplankton. However, the composition of bacterial communities in the frontal zone remains poorly understood. In this study, we investigate how a coastal tidal front in Brittany (France) structures the free-living bacterioplankton communities in a spatio-temporal survey across four cruises, five stations and three depths. We used 16S rRNA gene surveys to compare bacterial community structures across 134 seawater samples and defined groups of co-varying taxa (modules) exhibiting coherent ecological patterns across... |
| Tipo: Text |
Palavras-chave: Marine front; Bacterial communities; Dynamic; Network; Ecological strategies. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00634/74657/74557.pdf |
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| Frere, Laura; Maignien, Lois; Chalopin, Morgane; Huvet, Arnaud; Rinnert, Emmanuel; Morrison, Hilary; Kerninon, Sandrine; Cassone, Anne-laure; Lambert, Christophe; Reveillaud, Julie; Paul-pont, Ika. |
| Microplastics (<5 mm) exhibit intrinsic features such as density, hydrophobic surface, or high surface/volume ratio, that are known to promote microbial colonization and biofilm formation in marine ecosystems. Yet, a relatively low number of studies have investigated the nature of microplastic associated bacterial communities in coastal ecosystems and the potential factors influencing their composition and structure. Here, we characterized microplastics collected in the Bay of Brest by manual sorting followed by Raman spectroscopy and studied their associated bacterial assemblages using 16S amplicon high-throughput sequencing. Our methodology allowed discriminating polymer type (polyethylene, polypropylene and polystyrene) within small size ranges... |
| Tipo: Text |
Palavras-chave: Bacteria; Microplastics; Coastal ecosystem; Metabarcoding; Vibrios. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00449/56082/57614.pdf |
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| Dalmasso, Cecile; Oger, Philippe; Selva, Gwendoline; Courtine, Damien; L'Haridon, Stephane; Garlaschelli, Alexandre; Roussel, Erwan; Miyazaki, Junichi; Reveillaud, Julie; Jebbar, Mohamed; Takai, Ken; Maignien, Lois; Alain, Karine. |
| A novel strictly anaerobic, hyperthermophilic archaeon, designated strain CDGST, was isolated from a deep-sea hydrothermal vent in the Cayman Trough at 4964 m water depth. The novel isolate is obligate anaerobe and grows chemoorganoheterotrophically with stimulation of growth by sulphur containing compounds. Its growth is optimal at 75 °C, pH 6.0 and under a pressure of 50 MPa. It possesses the broadest hydrostatic pressure range for growth that has ever been described for a microorganism. Its genomic DNA G + C content is 51.11 mol%. The novel isolate belongs to the genus Thermococcus. Phylogenetic analyses indicated that it is most closely related to Thermococcus barossii DSM17882T based on its 16S rRNA gene sequence, and to ‘Thermococcus onnurineus’ NA1... |
| Tipo: Text |
Palavras-chave: Thermococcus; Piezophile; Hydrothermal vent; Cayman Trough. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00348/45949/45655.pdf |
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| Registros recuperados: 11 | |
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